Microsoft PowerPoint - Module_Mycotoxins_AK.pptx
Dieses Dokument ist Teil der Anfrage „Zwischenergebnisse und Empfehlungen der BfR-MEAL-Studie“
Design Module Mykotoxins 5th Advisory Board Meeting Anna Kolbaum, Dr. Irmela Sarvan, Dr. Oliver Lindtner Bundesinstitut für Risikobewertung
Outline 1. Look back: sampling year 1 ‒ Comparison of BfR-MEAL standard procedure with module „Mycotoxins“ ‒ Analytical results from sampling year 1 ‒ International comparison 2. Look forward: sampling years 2 and 3 Anna Kolbaum, 14.03.2019 5th Advisory Board Meeting BfR MEAL Study Seite 2
Look back Steps of the BfR MEAL Study Step Step11 Step 2 Step 3 Step 4 Step 5 Step 6 Selection of Shopping on Preparation and Pooling and Analysis Evaluation and foods national level processing homogenisation exposure assessment Anna Kolbaum, 14.03.2019 5th Advisory Board Meeting BfR MEAL Study page 3
Look back Selection of foods - Food List Step 1 CORE MODULE MYCOTOXINS • Main food groups (FoodEx2- • Main food groups (FoodEx2- Coding) Coding) 19 17 • Food groups in Mycotoxin • Food groups in BfR-MEAL Food List Food List 353 177 • Extended TDS-like approach • Pools, stratification: • regions • Pools, stratification: • Year-to-year variability (initial • Seasons (import/domestic) 846 • organic/conventional 531 planning) Anna Kolbaum, 14.03.2019 5th Advisory Board Meeting BfR MEAL Study Seite 4
TDS-standard procedure MYCOTOXINS TDS-Sampling Mycotoxin-Sampling - One piece or package per min. three pieces/packages, 1 2 3 Shopping subsample resulting in min. 1,5 kg per 1,5 kg subsample Reduced sampling for 1 2 3 selected foods ‚Standard‘ preparation of Simplified preparation 1 2 3 foods (one piece or package for selected foods (pre- Kitchen per subsample) mixing, pre-cutting) Pool Time for homogenisation Candied Fruits 2h 5h Dried fruits 4h 7h Pooling Raw ham 4h 7h Liquorice 2h 6h Anna Kolbaum, 14.03.2019 5th Advisory Board Meeting BfR MEAL Study Seite 5
Analysis Module „Mycotoxins“ Step 5 LOT 1 LOT 2 Analyte LOD LOQ Analyte LOD LOQ [µg/kg] [µg/kg] [µg/kg] [µg/kg] Type B Trichothecenes 7 20 Ergotalkaloids 0,7 2 (DON, NIV, 3-ADON, 15-ADON) Alternaria toxine (AME, 0,7 2 Zearalenon 2 5 AOH) Type A Trichothecenes T-2 Toxin 2 5 HT-2 Toxin 3 10 No offer for the analytes: Diacetoxyscirpenol 3 10 - DON-3-Glucoside Fumonisins (B1, B2) 3 10 - α-/β-Zearalenol - Sterigmatocystine Aflatoxins (B1, B2, G1, G2) 0,03 0,1 - Moniliformine Aflatoxin M1 0,003 0,01 - Other Alternaria toxins Ochratoxin A 0,07 0,2 Enniatine (A, A1, B, B1) 2 5 Beauvericin 0,7 2 Patulin 2 5 Citrinin 7 20 Anna Kolbaum, 14.03.2019 5th Advisory Board Meeting BfR MEAL Study Seite 6
Results of sampling year 1n – Module „Mycotoxins“ – Mycotoxins Anna Kolbaum, 14.03.2019 5th Advisory Board Meeting BfR MEAL Study Seite 7
Overview of results of the module „Mycotoxins“ from sampling year 1 Amount Percent Analytes, total 37 Pools, total 177 - Lot 1 - total 173 - Lot 2 - Ergotalkaloids 50 - Lot 2 - Alternaria 96 Analysis, total 4773 - Lot 1- total 3979 - Lot 2 - Ergotalklaoids 600 - Lot 2 - Alternaria 194 Detects, total* 128 3% - Lot 1 - total 115 3% * Amount of detects is based on an extended TDS-like approach - Lot 2 - Ergotalkaloids 9 2% (screening). Low amount of - Lot 2 - Alternaria 4 2% detects is expected. Anna Kolbaum, 14.03.2019 5th Advisory Board Meeting BfR MEAL Study Seite 8
Distribution of detects (n=115) over main food groups (%) in Lot 1 (food groups without detects not shown). Total amount of analysis in Lot 1: 3979. N: Amount of analysed pools per food group. 70% 62% n=71 60% 50% 40% 30% 20% 9% 7% n=10 n=8 3% 10% 3% 3% 2% 1% 1% 1% 1% n=4 n=3 n=3 n=2 n=1 n=1 n=1 n=1 0% Anna Kolbaum, 14.03.2019 5th Advisory Board Meeting BfR MEAL Study Seite 9
Distribution of detects (n=115) over analysed mycotoxins (%) in Lot 1 (Analytes without detects not shown). Total amount of analysis in Lot 1: 3979. 30% 24% 25% n=28 19% 20% n=22 16% n=18 15% 11% n=13 10% 7% 6% n=8 5% 5% n=7 n=6 n=6 5% 2% 2% 1% 1% 1% n=2 n=2 n=1 n=1 n=1 0% Anna Kolbaum, 14.03.2019 5th Advisory Board Meeting BfR MEAL Study Seite 10
